AURION Conventional Immunogold Reagents and Application Technique

AURION Conventional Immunogold Reagents and Application Technique

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Conventional Immunogold Reagents are available in four size classes. The monodisperse size population makes the conjugates suited for multiple labeling with no overlap. The Conventional Immunogold Reagents are the classical conjugates in immuno electron microscopy; they are a good choice when the antigen is abundant and the accessibility of the antigen is relatively good.

The conventional labeling approach in transmission and scanning electron microscopy utilizes secondary immunogold reagents based on particles that can be observed without enhancement. These conjugates are suited for single and multiple labeling in electron microscopy, when the number of antigens available for binding is such that a relevant signal can be obtained.

The AURION Conventional Immunogold Reagents are built around colloidal gold particles with sizes of 6, 10, 15 or 25 nm. The particle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6 and 25 nm particle size conjugates is less than 12%, whereas the 10 and 15 nm size conjugates show less then 10% variation.

The table below lists a few physical characteristics of gold conjugates:

6 nm 6500 1.3·10^6 2.4·10^13 1-2
10 nm 30·10^3 6·10^6 5·10^12 7-12
15 nm 100·10^3 20·10^6 1.5·10^12 25-40
25 nm 470·10^3 92·10^6 3.3·10^11 115-180

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