THE HIGHEST RESOLUTION AT EVERY OBJECTIVES
RAMANtouch/RAMANforce has finally accomplished perfect resolution.
Resolution at 20x and lower objectives has been improved to be optimal using a newly designed spectrograph without aberration, while maintaining the highest resolution with the 100x objective.
THE HIGHEST SPATIAL RESOLUTION
RAMANforce is equipped with dedicated optics and a high-quality TEM00 laser for the highest spatial resolution. With a 532 nm excitation wavelength and 0.90 NA (Numerical Aperture) objective, RAMANforce guarantees 350 nm spatial resolution for all users. Raman imaging data of fluorescent beads of 200 nm in diameter is shown on the right. The intensity profile along the dotted line in the Raman image demonstrates a spatial resolution of up to 300 nm. The high spatial resolution also enhances the detection sensitivity of tiny objects that are smaller than the diameter of the laser spot. RAMANforce is thus able to detect a small particle under 100 nm diameter.
Our highest spatial resolution and high performance confocal optics enhance our sensitivity to detect tiny particles. The laser beam will be accurately focused on the center of a particle using galvanometer scanner, and a robust sample stage keeps the laser spot always precisely located during long exposure measurements. RAMANforce produces a spectrum with a high signal-to-noise ratio that allows particle identification to be done by a library search.
ULTRA FAST RAMAN IMAGING BY LINE ILLUMINATION
The dedicated illumination mode developed by Nanophoton has dramatically improved the speed of Raman imaging by re-forming point laser beams into line shapes to cover a larger sample area. This patented line illumination excites Raman scattered light along the line-illuminated area simultaneously whereupon it is divided into 400 spectra by a large number of pixels using a CCD.
As one of Nanophoton’s specialized technologies RAMANtouch/RAMANforce is equipped with galvanometer scanner for fast and accurate laser beam scanning. Compared with a conventional motorized stage, laser beam scanning performs several hundreds times faster scanning with up to 10 nm positioning accuracy. This also has advantages in operation; e.g. just clicking on the microscopic image will designate the specific area for measurement without any vibrations.
There has been a long-lasting controversy about whether laser scanning technology deforms the laser spot, especially at the edge of the field-of-view. However, using the most updated optics allows focusing of the laser beam perpendicular to the focal plane over the whole area.