The AURION Blocking Solutions are used to prevent immunoreagents from binding non-specifically to specimens with “sticky” surface properties.
Procedures to eliminate background comprise three main steps:
- To suppress residual aldehyde activity
- To saturate multipoint hydrophobic moieties and positive charges with high molecular weight compounds such as those present in the AURION Blocking Solutions
- To reduce aspecific binding of immunoreagents caused by hydrophilic interaction with competing molecules in the incubation and washing solution. AURION BSA-c™ is a particularly effective reagent for this purpose.