AURION -- Reagents

AURION -- Reagents

Aurion, a Dutch company founded in 1990 is specialized in advanced techniques for electron microscopy and immunocytochemical detection systems for over twenty years. Quasi-S and Aurion has been partner since 2011 and provided a complete range of the best quality immunogold and auxiliary reagents to every needs and detection experiments. Among Aurion’s unique products is BSA-c™, an incubation buffer additive that has been developed for the elimination of background staining by charge specific competition, subnanometer ultra small gold reagents and R-Gent SE-EM silver enhancement reagents for Electron Microscopy.

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  • Blocking SolutionQuick view

    AURION Blocking Solutions

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    The AURION Blocking Solutions are used to prevent immunoreagents from binding non-specifically to specimens with “sticky” surface properties.

    Procedures to eliminate background comprise three main steps:

    • To suppress residual aldehyde activity
    • To saturate multipoint hydrophobic moieties and positive charges with high molecular weight compounds such as those present in the AURION Blocking Solutions
    • To reduce aspecific binding of immunoreagents caused by hydrophilic interaction with competing molecules in the incubation and washing solution. AURION BSA-c™ is a particularly effective reagent for this purpose.

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  • AURION BSA-c Incubation Solution AdditiveQuick view

    AURION BSA-c™ Incubation Solution Additive

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    AURION BSA-c™ is a buffer additive that helps prevent immunodetection reagents (i.e. primary antibodies and secondary reagents) from binding nonspecifically to charged moieties within the specimen. Thus, it suppresses background competitively with little or no effect on the specific reaction. Its successful application is not limited to immunogold detections but it is equally efficient in fluorescent and enzyme-based detection systems. AURION BSA-c™ concentrations as low as 0.01-0.1% inhibit binding of  gold conjugate to polycationic poly-l-lysine coated grids almost completely (>99%).

    • To suppress residual aldehyde activity
    • To saturate multipoint hydrophobic moieties and positive charges with high molecular weight compounds such as those present in the AURION Blocking Solutions
    • To reduce non-specific binding of immunoreagents caused by hydrophilic interaction with competing molecules in the incubation and washing solution.

    The surface properties of the specimen can be simplified by division into four compartments:
    - negatively charged (polyanions, proteins, especially after aldehyde fixation, lipids);
    0  neutral;
    + positively charged (histone proteins, polycations) and
    H hydrophobic (lipids, fat droplets, resins). After an appropriate blocking step these areas are covered with blocking compounds.

    AURION BSA


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  • Immunogold Reagents TechniqueQuick view

    AURION Conventional Immunogold Reagents and Application Technique

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    Conventional Immunogold Reagents are available in four size classes. The monodisperse size population makes the conjugates suited for multiple labeling with no overlap. The Conventional Immunogold Reagents are the classical conjugates in immuno electron microscopy; they are a good choice when the antigen is abundant and the accessibility of the antigen is relatively good.

    Download Application Technique below for more information.


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  • Starter KitsQuick view

    AURION Starter Kits

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    There are several kits available, containing either Conventional (gold particle sizes 6nm, 10 nm, 15 nm, 25 nm) or Ultra Small Gold Reagents (gold particle size < 0.8 nm), AURION BSA-c™ and silver enhancement reagents.

    Ultra Small-kit Electron Microscopy containing:

    • 2 small package vials Ultra Small ImmunoGold reagents of your choice
    • 30 ml AURION BSA-c™, an incubation buffer additive for the reduction of background staining
    • 30 ml AURION R-Gent SE-EM (silver enhancement reagents for EM)

    Ultra Small Kit Light Microscopy containing:

    • 2 small package vials Ultra Small ImmunoGold reagents of your choice
    • 30 ml AURION BSA-c™, an incubation buffer additive for the reduction of background staining
    • 60 ml AURION R-Gent SE-LM (silver enhancement reagents for LM)

    To make yourself familiar with our Ultra Small Gold Conjugates AURION has introduced the starter kit. This kit is sufficient for approximately 50 specimens.

    Ultra Small-starter kit containing:

    • 100 µl Ultra Small ImmunoGold reagent of your choice
    • 5 ml AURION R-Gent SE-LM (silver enhancement reagents for LM)
    • 3 ml AURION R-Gent SE-EM (silver enhancement reagents for EM)
    • 10 ml AURION BSA-c™, an incubation buffer additive for the reduction of background staining

    Conventional Kit containing:

    • 2 small package vials Conventional ImmunoGold reagents
    • 30 ml AURION BSA-c™, an incubation buffer additive for the reduction of background